The fraction of the lipid in a biological membrane that is in a fluid state: a spin label assay.

نویسندگان

  • H M McConnell
  • K L Wright
  • B G McFarland
چکیده

The solubility of the spin label TEMPO (I) in membranes depends on the fluidity of the membrane. An empir ical relat ionship has been establ ished between TEMPO solubility and fluidity, measured by the order parameter S~o. This relationship is employed to determine the fract ion of the lipid in membranes of rabbit muscle sarcoplasmic re t iculum that is in a fluid state. We find that 84% of the lipid in this membrane is in a fluid state at 25 °C.

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عنوان ژورنال:
  • Biochemical and biophysical research communications

دوره 47 1  شماره 

صفحات  -

تاریخ انتشار 1972